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Saturday, February 16, 2019

Difference between HPLC and GC

High-performance liquid chromatography and gas chromatography both are the separation techniques that are versatile, well-adapted, and have contributed to the growing scope of applications.
Here are mentioned some differences between the GC and HPLC.
High-performance liquid chromatography (HPLC):
  • In the HPLC system, the mobile phase is used as a liquid, such as methanol, water, and acetonitrile, etc.
  • HPLC uses UV, photodiode array detectors, RI, laser and conductivity detectors which are non-destructive.
  • Separations of analytes in HPLC generally carried out on the compounds that are higher molecular weights.
  • The liquid is used as a carrier, which is usually high viscosity, which increases the pressure in the HPLC system, hence the HPLC column is shorter and wider in diameter.
  • The retention time of the sample in the HPLC column depends on the difference of molecular size or polarity
  • HPLC analysis is generally done at ambient temperature.
Gas chromatography (GC):
  • Gas chromatography uses gas as a carrier, such as nitrogen, and helium.
  • GC uses a Flame ionization detector, FPD and NPD, which are destructive, Mass spectrometry (MS) detectors are a common detector for the liquid and gas chromatography.
  • Separations of analytes in GC generally carried out on the compound that’s few hundred molecular weights.
  • Gas chromatography uses gas as a carrier or mobile phase. It has less viscosity than HPLC mobile phase, therefore, the GC column which is long and narrow.
  • The retention time of the sample in the GC column relies on the difference in the volatility of analytes.
  • Gas chromatography analysis carried out at high temperatures.
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