The theoretical plate number is a
significant factor of the column; it is work as tool of column efficiency
measurement. Its illustrate the quality of separation and ability to generate
narrow and sharp peaks. Column having more number of theoretical pates are
consider as more efficient in separation than the plates with less number, hence the
peak resolution and narrow peak are depend upon the column efficiency.
N = 16(t/w)2
Hence
n = number of theoretical plates
t = retention time of analyte
w = width of the peak at base
The number of theoretical plates
is reliant on various parameters like analyte characteristics, flow rate, sample
size and temperature. As per the requirements theoretical plate number must be
more than 2000.
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