The performance of the HPLC column is a very significant factor in chromatography. Even enough care has been required to maintain column performance and life; however, you will not use the same as over a period of time. In many cases after over period of time various problems occur such as flaws appear in columns performance like build up pressure , split peaks, vary in peak area for same sample, vary in retention times, peak broadening, impurities deposits on the column inlet frit, therefore column loss his unique performance as well as integrity . Before the declaration of the column as the scrap, we need to require washing and significantly regenerating and enhancing the column performance properly.
For the regeneration of the column, placed the position in opposite direction. For reveres, phase columns like C8 and C18 flush with adequate time as per listed solvents according to polarity index. And same procedure is followed in reverse chronological order for regeneration of column.
Water: Methanol 50:50 %
Methanol 100 %
Methanol: Acetonitrile 50:50%
Acetonitrile 100%
Acetonitrile: THF 50:50 %
THF 100 %
THF: Hexane 50:50 %
Hexane 100 %
It is recommended you to follow the column user guide provided by the manufacturer.
| You may also like this HPLC Columns HPLC column care and maintenance Advantages of HPLC Retention Time Factor Affecting Retention Time in HPLC Resolution in Chromatography Dead Volume |
No comments:
Post a Comment